Welcome to the Cerebellar Gene Regulation in Space & Time Database
Overview
Microarray
The Cb GRiTS Database resource represents a growing number of datasets for exploring
cerebellar development of the mouse in time and space.
The database contains a number of mutant/wildtype microarray datasets including
two complete wildtype microarray time-series (C57BL/6 and DBA/2J). Our time-series
data spans twelve time-points between E12-P9. The dataset also includes in
situ hybridization and bioinformatic analyses.
In Situ Hybridization
Exploration of this dataset will allow the investigator to assess differential gene
expression profiles from a developing mutant cerebella, to assess the temporal changes
in gene expression in the wildtype, and to verify the cellular expression of these
genes in images from our in situ hybridization library.
Bioinformatic Analysis
Using our database, the investigator can explore the developmental expression or
differential expression patterns of a particular gene, or create lists of similarly
expression genes by building simple search algorithms. These lists can then
be mined accross all the datasets in both space and time.
Current Datasets
Our current datasets represent gene expression analyses from multiple cerebellar
mutant and wildtype single time-point and developmental series.
- C57BL/6 WT Time Series - A developmental time-series (E12,
E13, E14, E15, E16, E17, E18, E19, P0, P3, P6, P9) from wildtype C57BL/6 mice.
Run on Illumina v1.1 platforms. [Goldowitz Lab, CMMT]
- DBA/2J WT Time Series - A developmental time-series (E12,
E13, E14, E15, E16, E17, E18, P0, P3, P6, P9) from wildtype DBA/2J mice. Run
on Illumina v1.1 platforms. [Goldowitz Lab, CMMT]
- Math1 Mutant (E15.5) - A single time-point (E15.5) from
mutant and wildtype littermates carrying the Rhombic lip derivative gene, Math1.
Run on an Affymetrix U74a_v2 and M430A&B platforms. [Goldowitz Lab, UTHSC]
- Meandertail Mutant - A single time-point (E15.5) from mutant
and wildtype littermates carrying the presumed granule cell specific, "Meandertail,"
gene mutation. Run on an Affymetrix M430v2.0 platform. [Goldowitz Lab, UTHSC]
- Pax6 Sey Mutant Time Series - A developmental time-series
(E13.5, 15.5, 18.5) from mutant and wildtype littermates carrying the cerebellar
granule cell specific gene, Pax6, gene null mutation. Run on an Affymetrix U74a_v2
platform. [Goldowitz Lab, UTHSC]
- Riken Mut/WT Time Series - A developmental time-series
(E18.5, P7, P14, P21, P56) from wildtype C57BL/6 mice. Run on the Affymetrix MU11K
platform. [Teiichi Furuichi, Riken Institute]
- Staggerer Mut/WT Time Series - A development time series
(E15.5, E17.5, P0, P1, P4) from mutant and wildtype littermates carrying the Purkinje
cell specific, RORa, knockout mutation. Run on an Affymetrix U74a_v2 platform. [Bruce
Hamilton, UCSD]
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Zic 1/4 Mut/WT Time Series
Future additions to the database will include a developmental series from BXD RI
lines and an extensive survey of additional cerebellar mutant lines.
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